Intermediate

Part:BBa_K133012:Design

Designed by: Ana Lasic   Group: iGEM08_Slovenia   (2008-10-29)

CF213-UreB-linker-CF215-RGD-Histop


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2958
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1655
    Illegal BamHI site found at 2515
    Illegal BamHI site found at 3026
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 301
    Illegal AgeI site found at 877
    Illegal AgeI site found at 2055
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 3309
    Illegal SapI.rc site found at 763


Design Notes

The part was cloned into pSB1.AK3 without terminator for use in bacterial cells (surface expression).

Source

plasmid DNA (our other parts)

References